 =Rhizoglyphus robini

 PoloPlus  Version 2.0
 Date: 30 APR 2026
 Rhizoglyphus robini                                                     
 Data file: C:\Users\Hosein\Desktop\Aba+Nickle.txt
 Number of preparations: 2
 Number of dose groups: 32
 Model: Probit
 Natural Response Parameter: no
 Convert doses to logarithms: yes
 LDs:  50
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 Rhizoglyphus robini                                                     
  
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 Intercepts and slopes unconstrained. Preparation is ( 1) Aba     
 Not estimating natural response
  
             parameter   standard error   t ratio
 Aba          -1.425          0.169       -8.425
 SLOPE         3.467          0.345       10.044
  
 Variance-Covariance matrix
                 Aba           SLOPE       
   Aba         0.285887E-01 -0.543269E-01
   SLOPE      -0.543269E-01  0.119121    
  
 Chi-squared goodness of fit test
 prep             dose      n       r   expected  residual   probab  std resid
 Aba             5.400     32.     28.     27.76     0.241    0.867     0.126
                 5.400     30.     25.     26.02    -1.024    0.867    -0.551
                 5.400     25.     22.     21.69     0.313    0.867     0.185
                 5.400     28.     24.     24.29    -0.289    0.867    -0.161
                 3.600     26.     18.     18.01    -0.015    0.693    -0.006
                 3.600     34.     24.     23.56     0.442    0.693     0.164
                 3.600     42.     28.     29.10    -1.101    0.693    -0.368
                 3.600     31.     22.     21.48     0.521    0.693     0.203
                 2.400     35.     17.     16.02     0.983    0.458     0.334
                 2.400     35.     16.     16.02    -0.017    0.458    -0.006
                 2.400     30.     15.     13.73     1.271    0.458     0.466
                 2.400     29.     14.     13.27     0.729    0.458     0.272
                 1.600     27.      6.      6.39    -0.391    0.237    -0.177
                 1.600     25.      5.      5.92    -0.918    0.237    -0.432
                 1.600     28.      6.      6.63    -0.628    0.237    -0.279
                 1.600     29.      7.      6.86     0.135    0.237     0.059
  
 chi-square: 1.285     degrees of freedom: 14     heterogeneity: 0.092
  
 Effective Doses
                   dose      limits    0.90     0.95     0.99
 LD50  Aba        2.576      lower    2.393    2.357    2.284
                             upper    2.756    2.792    2.863
  
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 Intercepts and slopes unconstrained. Preparation is ( 2) Aba+Nick
 Not estimating natural response
  
             parameter   standard error   t ratio
 Aba+Nick     -1.428          0.194       -7.369
 SLOPE         3.016          0.354        8.524
  
 Variance-Covariance matrix
                 Aba+Nick      SLOPE       
   Aba+Nick    0.375638E-01 -0.645347E-01
   SLOPE      -0.645347E-01  0.125236    
  
 Chi-squared goodness of fit test
 prep             dose      n       r   expected  residual   probab  std resid
 Aba+Nick        6.000     30.     23.     24.63    -1.629    0.821    -0.776
                 6.000     27.     21.     22.17    -1.166    0.821    -0.585
                 6.000     28.     23.     22.99     0.013    0.821     0.006
                 6.000     21.     16.     17.24    -1.240    0.821    -0.706
                 4.000     23.     17.     14.97     2.028    0.651     0.887
                 4.000     31.     22.     20.18     1.821    0.651     0.686
                 4.000     28.     20.     18.23     1.773    0.651     0.703
                 4.000     27.     19.     17.58     1.424    0.651     0.575
                 2.700     24.     11.     10.79     0.213    0.449     0.087
                 2.700     30.     13.     13.48    -0.484    0.449    -0.178
                 2.700     25.     11.     11.24    -0.237    0.449    -0.095
                 2.700     23.     10.     10.34    -0.338    0.449    -0.142
                 1.800     22.      5.      5.61    -0.615    0.255    -0.301
                 1.800     27.      7.      6.89     0.109    0.255     0.048
                 1.800     25.      6.      6.38    -0.380    0.255    -0.175
                 1.800     23.      5.      5.87    -0.870    0.255    -0.416
  
 chi-square: 3.890     degrees of freedom: 14     heterogeneity: 0.278
  
 Effective Doses
                   dose      limits    0.90     0.95     0.99
 LD50  Aba+Nick   2.975      lower    2.719    2.668    2.564
                             upper    3.231    3.282    3.386
  
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 HYPOTHESIS OF EQUALITY (equal slopes, equal intercepts): REJECTED (P<0.05)
    (chi-square: 6.82,  degrees of freedom: 2,  tail probability: 0.033)
  
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 HYPOTHESIS OF PARALLELISM (equal slopes): NOT REJECTED (P>0.05)
    (chi-square: 0.83,  degrees of freedom: 1,  tail probability: 0.362)
  
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 Lethal dose ratio (LD50)
              ratio      limits     0.95
 Aba+Nick     0.866      lower     0.758
                         upper     0.989
  
